The yeast community associated to bark beetles is unknown. Recently, it has been demonstrated that some yeast species of the alimentary canal and frass of Dendroctonus genus can help in the detoxification process and pheromone production. In this study we reported and characterized 450 strains isolated from gut, ovaries, eggs and frass of nine Dendroctonus species from 36 geographical sites. We used 40 physiology tests and partial sequences of D1/D2 26S, 18S e ITS1 from rDNA to taxonomic identification of strains. Phylogenetic reconstruction methods used for these three gene shown 13 consistent groups associated to Candida arabinofermentans, C. ernobii, C. oregonensis, C. picea, C. terebra, Pichia americana, P. canadensis, P. capsulate, P. glucozyma, P. guilliermondii, P. scolyti and other two that have not equivalent within the GenBank database.