The Colorado potato beetle, Leptinotarsa decemlineata Say, is the major insect pest of potato (Solanum tuberosum L.) in eastern North America and is renowned for insecticide resistance development. Host plant resistance could be an important management tool. A no-choice cage study was conducted to evaluate natural host plant resistance mechanisms (glandular trichomes and Solanum chacoense-derived glycoalkaloid-based resistance) and engineered (Bt-cry3A and Bt-cry1Ia1) for control. The no-choice situation attempts to simulate a monoculture environment typical of growers’ fields. Six different potato clones representing four different host plant resistance mechanisms were evaluated for two years. Ten egg masses were placed in each cage (1 egg mass/plant). Little or no feeding was observed in the Bt-cry3A and Bt-cry1Ia1 lines. In 2003, the glycoalkaloid line experienced less defoliation than either the control or the glandular trichome line; in 2004, the glycoalkaloid line had more defoliation than the control or glandular trichome line. Defoliation in the glycoalkaloid line was largely due to larvae clipping the petioles, rather than direct consumption. Defoliation in the glandular trichome line did not significantly differ from the control, suggesting glandular trichomes may not be effective in a no-choice situation. The results from this study may be used to assess host plant resistance mechanisms for control of Colorado potato beetle larvae.