The 2005 ESA Annual Meeting and Exhibition
December 15-18, 2005
Ft. Lauderdale, FL

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Friday, December 16, 2005

Analysis of the microbiota of the gut of Dendroctonus spp. (Coleoptera: Curculionidae:Scolytinae)

FJavier Zavala Diaz de la Serna,, Escuela Nacional de Ciencias Biologicas, Microbiology, Prol. de Carpio y Plan de Ayala s/n. Col. Santo Tomas, México D. F, Mexico, Gerardo Zúñiga,, Instituto Politécnico Nacional, Departamento de Zoología, Prol. de Carpio y Plan de Ayala s/n. Col. Santo Tomas, México D. F, Mexico, and César H. Hernández,, Instituto Politécnico Nacional, Departamento de Microbiología, Prol. de Carpio y Plan de Ayala s/n. Col. Santo Tomas, México D. F, Mexico.

Bark beetles of the genus Dendroctonus are a group of insects widely distributed in North American and Eurasia. These bark beetles feed on phloem and vascular cambium from coniferous trees. They maintain symbiotic associations complex with bacterial and fungal. In this work, the microbial diversity associated to the gut of bark beetles was studied. Guts from D. pseudotsugae, D. adjuntus; D. approximatus, D. valens were collected from several geographical sites in Mexico. The gut was obtained and sectioned in foregut, midgut, and hindgut. The DNA of these three region was used to amplify the 16S and 26S ribosomal genes with the purpose to evaluate bacterial and fungal communities by PCR-DGGE procedures. In the species studied were observed different microbial composition between foregut and midgut-hindgut. A clear difference was observed among bacterial and fungal communities of Dendroctonus species. Additionally, DGGE analysis revealed common amplification bands with previously cultured yeast, but other amplification bands had not relationship with any isolated strains. Particularly The 16S and 26S bacterial libraries and RFLP analysis from gut of D. pseudotsugae revealed limited ascomycete and bacterial diversity.

Species 1: Coleoptera Curculionidae Dendroctonus
Keywords: Microbial communities, Bark beetles

Poster (.pdf format, 308.0 kb)