In laboratory bioassays Steinernema riobrave Cabanillas, Poinar and Raulston, S. carpocapsae (Weiser) (Mexican 33), Steinernema feltiae (Filipjev) (UK76), and Heterorhabditis bacteriophora Poinar (HP88) were all capable of infecting and killing three termite species, Heterotermes aureus (Snyder), Gnathamitermes perplexus (Banks), and Reticulitermes flavipes (Kollar). Only S. riobrave and S. feltiae caused any mortality of Reticulitermes virginicus (Banks). At 22 degree Celsius, significant mortality (over 80%) of H. aureus and G. perplexus was caused by S. riobrave, indicating the potential role of these parasitoids as a biocontrol option. Due to the short assay time (3 days maximum) reproduction of the nematodes in the target host species was not observed but all nematode species were observed to develop to fourth-stage juveniles, pre-adult stages or adults in all termite species with the exception of R. virginicus. Only S. riobrave developed in R. virginicus. Both nematodes dose rates and incubation time have significant effect on the mortality of H. aureus.