Extensive research has been conducted on the optimum nitrogen response of corn, Zea mays L., to nitrogen fertilizer under a variety of environmental conditions. To date, this research has focused on non-transgenic corn plants. Transgenic corn rootworm hybrids are not expected to have measurable root pruning; therefore, transgenic corn rootworm hybrids may be able to utilize nitrogen more efficiently. Improving our knowledge of nitrogen requirements of transgenic corn plants may aid in improving our nitrogen fertility recommendations to producers. The objective of this study is to evaluate corn rootworm larval injury, plant development, nitrogen utilization, and yield of a transgenic corn rootworm hybrid (MON 863, expressing the Cry 3Bb1 protein) and its non-transgenic isoline at several nitrogen rates, with and without a systemic insecticidal seed treatment (clothianidin). Preliminary research was conducted in 2004 at the Monsanto Research Farm in Monmouth, IL, in continuous corn. Results indicated that the transgenic corn rootworm hybrid had less root injury, less lodging, and greater yield. Also, significantly more nitrogen uptake occurred in the transgenic corn rootworm hybrid than the insecticide treated and untreated non-transgenic isolines.