Watanaporn Dheranetra, watanaporn.dheranetra@na.amedd.army.mil1, John Paul Benante, john.benante@na.amedd.army.mil1, Mark A. Potter, mark.potter@na.amedd.army.mil1, Daniel A. Nichols, daniel.nichols@na.amedd.army.mil2, Apurba K. Bhattacharjee, apurba.bhattacharjee@na.amedd.army.mil2, Bryan Mott, apurba.Bhattacharjee@na.amedd.army.mil2, Charles E. White, charles.white@na.amedd.army.mil3, Nancy McLean-Cooper, nancy.mclean-cooper@na.amedd.army.mil1, and Raj K. Gupta, raj.gupta@amedd.army.mil4. (1) Walter Reed Army Institute of Research, Department of Entomology, 503 Robert Grant Avenue, Silver Spring, MD, (2) Walter Reed Army Institute of Research, Department of Medicinal Chemistry, 503 Robert Grant Avenue, Silver Spring, MD, (3) Walter Reed Army Institute of Research, Department of Biostatistics, 503 Robert Grant Avenue, Silver Spring, MD, (4) US Army, HQ, US Army Medical Research and Materiel Command, 504 Scott St, MCMR-PR, Fort Detrick, MD
Four different membranes (Baudruche, Hemotek, sausage, and silicone) were evaluated as human skin substitute for in vitro repellent evaluations using Aedes aegypti. ANOVA analysis indicated no significant difference in repellency activity (ED50s). Sausage membrane was selected to replace Baudruche membrane due to its availability, simple application technique, ease of use and low cost. We evaluated ten new custom synthesized compounds based on our previously reported in silico 3D pharmacophore model for repellency against Aedes aegypti using the in-vitro test system. Seven of these new compounds showed good repellency (G<1) with ED50 values ranging from 0.001575 mg/cm2 to 0.102891 mg/cm2 and ED95 ranged from 0.614490 mg/cm2 to 1.203814 mg/cm2, respectively. The new compounds are being further explored for their potential repellent activity.
Species 1: Diptera Culicidae
Aedes aegyptiKeywords: new insect repellent compounds, Comparison of four different membranes
Poster (.pdf format, 760.0 kb)
