Road construction may have potential harmful effects on headwater stream communities. The purpose of this study was to examine the effects of road construction on macroinvertebrate communities. Data collected from 1998 to 2000 monitoring periods represent aquatic conditions prior to the commencement of highway construction. Eight specific locations were selected as the primary monitoring stations down gradient of potential impact areas from the proposed highway alignment. Additional primary monitoring stations were added to examine potential impacts downslope of the mountain ridge side and the headwater tributaries. Biological monitoring was conducted at each station utilizing the inhabitant benthic macroinvertebrate communities as indicators of short-and long-term water quality conditions. Three benthic macroinvertebrate subsamples were conducted at each primary monitoring station during the spring and fall field investigations using a rectangular frame kicknet. Ten metrics associated with macroinvertebrate community, population, and functional structure were evaluated to assess the condition of the biological conditions. Metric analysis completed during the pre-construction sampling events were compared with subsequent sampling events during active construction. The active construction sampling results indicate that the aquatic resources remain to be of reputable quality. All primary monitoring stations are characterized by a high degree of biological integrity and do not appear impaired due to highway construction.