Cotesia is a genus of parasitoid wasps that has been widely used for biological control of insect pests. The Cotesia flavipes complex contains three species, C. flavipes, C. sesamiae and C. chilonis, which are difficult to distinguish morphologically. Cotesia flavipes is distributed throughout the Indo-Australian region, C. sesamiae is found in Africa, and C. chilonis has been collected from Japan and China. These parasitoids have been used for biological control in over 40 countries for stem boring lepidopteran pests such as Chilo and Diatraea spp., which damage crops including rice, sugar cane, maize and sorghum. Our objective was to determine if courtship signals differed among the three members of this species complex. Courtship songs have been employed successfully to distinguish among morphologically similar insects in orders such as Orthoptera, Neuroptera and Hemiptera. Male Cotesia species wing fan while courting females, and wing fanning produces a low amplitude song. The courtship songs of males of all three species of the C. flavipes complex were recorded, as was a more distantly related species, Cotesia marginiventris. Signal analysis was conducted to determine the pulse duration, pulse frequency, and interpulse intervals from the recordings, and to characterize the signals of the different species.