The behavior of neonate grape berry moth, Endopiza viteana, was observed during selection and acceptance of entry sites on mature grape berries. This revealed a sequence of behaviors, from movement and searching behaviors associated with entry site selection, to behaviors associated with berry penetration. Larvae engaged in feeding behaviors 50% of the time, and spent 60% of their time at the collar region near the stem, where the majority of berry entries occurred. Excavation of penetration sites started within 40 minutes of placement on berries and larvae had entered the berry tissues within 120 minutes of placement. Larval behavior was also compared among untreated grapes or those treated with methoxyfenozide, spinosad, or acetamiprid, revealing significant disruption of typical behaviors and variability among treatments. The implications for preventing berry penetration by this important vineyard pest will be discussed.