The 2005 ESA Annual Meeting and Exhibition
December 15-18, 2005
Ft. Lauderdale, FL

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Saturday, December 17, 2005 - 8:12 AM
0784

A comparison of DNA profiling of human blood meals in Aedes and Anopheles

Thomas W. Scott, twscott@ucdavis.edu, University of California at Davis, Department of Entomology, Davis, CA, Laura C. Harrington, Ich27@cornell.edu, Cornell University, Department of Entomology, Ithaca, NY, James Winifred Jones, james.winifred.jones@us.army.mil, Armed Forces Research Institute of Medical Sciences, Department of Entomology, US Army Medical Component, AFRIMS, Apo, AP, Thailand, Sangvorn Kitthawee, grskt@mucc.mahidol.ac.th, Mahidol University, Deaprtment of Biology, Rama VI Road, Bangkok, Thailand, Ratana Sithiprasasna, ratanas@afrims.org, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Department of Entomology, 315/6 Rajvithi Road, Bangkok, Thailand, Kriangkri Lerdthusnee, KriangkraiL@THAI.AMEDD.ARMY.MIL, AFRIMS, Entomology, 0000 Road, Bangkok, Thailand, John D. Edman, jdedman@ucdavis.edu, University of California, Department of Entomology, Davis, CA, Russell Coleman, russell.coleman@na.amedd.army.mil, U. S. Army, Walter Reed Army Institute of Research, WRAIR, Department of Entomology, 503 Robert Grant Ave, Silver Spring, MD, Andrew Githeko, AGitheko@kisian.mimcom.net, Kenya Medical Research Institute, KMRI, Kisumu, Kenya, and Guiyun Yan, gyan@acsu.buffalo.edu, The State University of New York, Biological Sciences, Buffalo, NY.

We used PCR-based DNA profiling to reconstruct human-mosquito interactions in Thailand, Puerto Rico, and Kenya. Using hypervariable human genetic markers we determined the person(s) bitten and, thus, the distribution of vector bites across people of different age, sex, and residence status. Epidemiological implications of feeding patterns will be discussed and compared across the species and geographic locations studied.


Species 1: Diptera Culicidae Aedes aegypti (yellow fever mosquito)
Species 2: Diptera Culicidae Anopheles gambiae
Species 3: Diptera Culicidae Anopheles funestus
Keywords: DNA fingerprinting, mosquitoes