The USDA APHIS PPQ CPHST, Pest Detection, Diagnostics, and Management Laboratory (PDDML) in Edinburg, TX, mass produces a culture of Cyrtobagous salviniae (Coleoptera: Curculionidae) for biological control of Salvinia molesta, giant salvinia (GS). The C. salviniae culture was established in 2000 from collections in Australian sites where introductions of Brazilian weevils led to a successful GS biological control in Australia during the 1980’s. Following completion of the required host specificity testing and receipt of an approved US field release permit, C. salviniae releases were initiated in October 2001 in 6 evaluation sites in east Texas. Additional releases and monitoring continued throughout the next 2 years that led to weevil establishment and successful control of giant salvinia in 5 of the 6 sites. The GS biological control program expanded to include establishment of field insectary sites (FIS) in other states from which within-state redistribution of C. salviniae could occur. Nine (9) FIS were successfully established in Louisiana, North Carolina, and north Texas, and releases were initiated in April 2005 in Mississippi. Transfer of the GS biological control program technology will continue through 2006 with survey, site identification and/or releases of C. salviniae in Alabama, Florida, Georgia, Puerto Rico, South Carolina, and Virginia. In addition, PDDML continues to supply C. salviniae for a similar release program in Arizona and California conducted by another CPHST laboratory located in Brawley, CA, and for release by other cooperators; and for support of the Hawaiian host specificity testing program.