The 2005 ESA Annual Meeting and Exhibition
December 15-18, 2005
Ft. Lauderdale, FL

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Sunday, December 18, 2005
D0526

Multiple displacement amplification in combination with high-fidelity PCR improves detection of bacteria from single females or eggs of Metaseiulus occidentalis (Nesbitt) (Acari: Phytoseiidae)

Ayyamperumal Jeyaprakash, Ajey@mail.ifas.ufl.edu and Marjorie A. Hoy, mahoy@ifas.ufl.edu. University of Florida, Entomology and Nematology, 970 Natural Area Drive, Gainesville, FL

Amplifying microbial DNA by polymerase chain reaction (PCR) from single phytoseiid mites has been difficult, perhaps due to interference by relatively large amounts of mite genomic DNA. A multiple displacement (MDA) procedure in combination with high-fidelity PCR amplified bacterial DNA successfully from single females or eggs of Metaseiulus occidentalis. The efficiency of this protocol to amplify bacterial DNA was estimated and presented.


Species 1: Acari Phytoseiidae Metaseiulus occidentalis (Western predatory mite)
Keywords: Multiple displacement amplification, High-fidelity PCR

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