Tuesday, 16 November 2004

Temporal expression of DlEPV structural proteins in host hemocytes

Pauline O. Lawrence, pol@ifas.ufl.edu, University of Florida, Entomology and Nematology, 970 Natural Area Drive, Gainesville, FL

The Diachasmimorpha longicaudata entomopoxvirus (DlEPV) is introduced into the Anastrepha suspensa larval host during oviposition by the D. longicaudata female wasp. The virus invades host hemocytes, replicates, and extracellular enveloped virus (EEV) bud into the hemolymph to infect new cells. This study was designed to determine the hours post parasitism (hpp) when DlEPV structural proteins first appear. These proteins are indicative of the onset of late gene expression and are concomitant with viral assembly. Using an anti-DlEPV serum, 1-D and 2-D gel electrophoresis, and Edman degradation sequencing, we determined that two major 70 and 74 kDa proteins first appear in hemocytes within 48 hpp. The smaller protein has high homology with poxvirus viral membrane proteins while the latter was highly phosphorylated but had no homologs in the database. These results suggest that the virus quickly establishes within the host and induces blebbing, apoptosis, and inhibition of encapsulation. Funded by NSF grant 9986076 to POL.

Species 1: Hymenoptera Braconidae Diachasmimorpha longicaudata
Species 2: Diptera Tephritidae Anastrepha suspensa (Caribbean fruit fly)
Keywords: entomopoxvirus, structural proteins

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