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Tuesday, 16 November 2004
D0404
Deciphering the herbivore-induced transcriptomes of Nicotiana attenuata and Solanum nigrum
Claudia Voelckel, voelckel@ice.mpg.de1, Dominik D. Schmidt2, and Ian Baldwin, Baldwin@ice.mpg.de1. (1) Max Planck Institute for Chemical Ecology, Department of Molecular Biology, Hans-Knöll-Str. 8, Beutenberg Campus, Jena, Germany, (2) John Innes Center, Department of Disease and Stress Biology, Norwich Researc Park, Colney, Norwich, United Kingdom
We applied large scale transcriptional profiling using the TIGR 10,000 unigene cDNA potato array to explore two different aspects of specificity in plant-insect interactions. First, we compared transcriptional responses in N. attenuata (coyote tobacco) and Solanum nigrum (black nightshade) when challenged with the Solanaceous generalist M. sexta. Differences were not only found for the elicitation of secondary metabolism genes but also for signaling pathway genes contradicting the idea of a ‘blueprint’ of defense mechanisms even in closely related species. In a second comparison, we monitored the transcriptional responses of N. attenuata to the attack of two native and highly abundant herbivores – leaf-chewing Manduca sexta larvae and mesophyll-sucking Tupiocoris notatus bugs. Data mining using principal components- and hierarchical cluster analyses revealed a) the elicitation of herbivore-specific imprints after 24h of attack, b) the transience of responses and thus the importance of considering kinetics when studying specificity, d) a specific imprint upon stress combination and e) PCA to be the more informative approach compared to hierarchical clustering. The results from these explorative analyses greatly enhanced our understanding of transcriptional plasticity in N. attenuata and S. nigrum and are a prerequisite for the design of boutique arrays for both species.
Species 1: Lepidoptera Sphingidae Manduca sexta
Keywords: host-plant resistance, induced responses
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