An immuno-marking system was developed that allows the treatment of large areas with inexpensive and readily available protein sources that can be detected at low levels using commercially available antibodies. We have developed and tested ELISA assays against egg albumin, casein, and soy protein. There is no cross-reaction between the different assays and each insect sample can be screened separately against the other assays so that the system can be used to determine inter-area movement patterns. We tested the assays against several common agricultural adjuvants, examined the different methods by which the mark could be acquired, and examined longevity in field trials.