The western corn rootworm (WCR), Diabrotica virgifera virgifera, has been able to circumvent crop rotation in east-central Illinois and northern Indiana. Females oviposit in soybean fields that will be planted to corn the following spring. Separation of the wild type and rotation resistant phenotypes is important to understanding the genetic basis of this selection and to determine which areas will be most susceptible to damage by rotation resistant WCR. This study examined female movement as a means to separate the two phenotypes. A previous study had suggested that response to a pyrethroid insecticide would elicit a difference in behavior, but we found that activity levels were different with no stimulus. Beetles from the rotation resistant population were more active, which may be driving them to leave natal cornfields to oviposit in soybean fields. Results will be reported for two summers’ work on a behavioral bioassay to differentiate rotation resistant beetles from the wild type.