Sunday, 14 November 2004 - 8:00 AM

Genetic evaluation of alfalfa weevil, Hypera postica, populations in New Mexico

Paul F. Smith,, C. Scott Bundy,, Steve Hanson,, and Dennis Sutton, New Mexico State University, Entomology Plant Pathology and Weed Science, Skeen Hall Rm. N141, Las Cruces, NM

The alfalfa weevil, Hypera postica (Gyllenhal), is a serious pest of alfalfa throughout the United States. Three strains of alfalfa weevil (eastern, western and Egyptian) are found in the United States, and are thought to have populations that meet or overlap in New Mexico. The purpose of our study is to genetically evaluate several alfalfa weevil populations throughout the state, to determine what strains are present, and if different strains have overlapping populations. Restriction digestion of PCR products and direct sequencing of weevil DNA was done to determine the identity of alfalfa weevil populations from five locations throughout the state of New Mexico (Las Cruces, Los Lunas, Artesia, Farmington, and Lordsburg). Fifty individuals were collected from each site, and DNA was extracted and analyzed. Initial results show that all three strains are found in the state, and that the eastern and western strains, and the eastern and Egyptians strains of the weevil have overlapping populations.

Species 1: Coleoptera Curculionidae Hypera postica (Alfalfa Weevil)
Species 2: Fabaceae Medicago sativa (alfalfa)
Keywords: RFLP, genetics

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