Tuesday, 16 November 2004

Genetic transformation of the codling moth, Cydia pomonella, with piggyBac EGFP

Holly J. Ferguson, hferguson@wsu.edu1, Lisa G. Neven, neven@yarl.ars.usda.gov2, Douglas B. Walsh, dwalsh@wsu.edu1, Stephen Thibault, thibault@exelixis.com3, and John J. Peloquin, peloquin@faculty.ucr.edu4. (1) Washington State University, Entomology, 24106 N. Bunn Rd, Prosser, WA, (2) Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Road, Wapato, WA, (3) Exelixis Pharmaceuticals, 170 Harbor Way, South San Francisco, CA, (4) University of California-Riverside, Biochemistry, Department of Biochemistry, Riverside, CA

There has been sustained interest in genetically transforming the codling moth, Cydia pomonella, over the past ten years. The main motivation is to develop a conditionally sterile mutant to be used in a sterile insect technique program, thereby eliminating the traditionally used sterilizing irradiation that is costly in terms of labor, expense, and moth fitness. piggyBac transposon mobility assays and egg microinjection experiments indicated that the piggyBac vector containing enhanced green fluorescent protein (EGFP) would be suitable as a transformation vector in codling moth. Data are presented here to show the successful introduction and inheritance of the EGFP gene, using a plasmid piggyBac vector. No evidence of stable integration was obtained with the piggyBac vector though GFP-positive moths as far out as generation 14 were detected, suggesting that the introduced EGFP gene maintained association with codling moth genomic DNA over the long term.

Species 1: Lepidoptera Tortricidae Cydia pomonella (codling moth)
Keywords: genetic transformation, piggyBac

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