Tuesday, 16 November 2004

Genetic variability of mitochondrial DNA in screwworm fly from Uruguay

Mariana Lucio Lyra, marilyra@unicamp.br1, Pablo Fresia, fresia@fcien.edu.uy2, Juan Cristina2, Louis Bernard Klaczko, lbk@unicamp.br1, and Ana Maria Lima de Azeredo-Espin, azeredo@unicamp.br1. (1) Universidade Estadual de Campinas, Laboratório de Genética Animal, CBMEG, Campinas, Sao Paulo, Brazil, (2) Universidad de la República, Facultad de Ciencias, Montevideo, Montevideo, Uruguay

The primary screwworm fly Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) is one of the most important insect pests of livestock in neotropical regions. In this work, PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) of mitochondrial DNA (mtDNA) was used to study the diversity and population structure of seven geographic populations from Uruguay. The Control Region and the subunits I and II of Cytochrome Oxidase were amplified and digested with diagnostics enzymes. Nine haplotypes were observed among the C. hominivorax samples examined. Mean nucleotide diversity (π=2,3%) and haplotype diversity (H=0,6533) indicated high mtDNA variability in this specie. The similarity index (96,7%), the nucleotide divergence (d=0,00055) and the AMOVA result showed no evidence of subpopulation differentiation, indicating that the populations of Uruguay are a unique panmitic population. The characterization of NWS intra specific genetic variability at the geographic level is necessary to monitoring the expansion in the current distribution and into screw-worm free areas. Financial support: FAPESP, CNPq, IAEA.

Species 1: Diptera Calliphoridae Cochliomyia hominivorax (screw-worm fly)
Keywords: mtDNA, PCR-RFLP

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