Monday, November 15, 2004
0094

A protocol for using the mite brushing machine (Leedom Industries) for measuring levels of Willamette spider mite (Eotetranychus willamettei McGregor) and their predators (Phytoseiidae) on winegrapes

Craig W. Macmillan, cwmacmil@calpoly.edu and Michael J. Costello, mcostell@calpoly.edu. California Polytechnic State University, Horticulture & Crop Science, San Luis Obispo, CA

Enumerative methods for estimating field populations of arthropod pests are accurate and precise, but time-consuming. The Mite Brushing Machine is a technology that can reduce the time required to obtain either absolute counts or estimates of arthropods on leaves from samples. Although this technology was investigated and recommended as a useful tool for research and commercial pest management by research entomologists and the USDA, little work to demonstrate the validity of this technique has been performed since the 1950’s.

The areas of this investigation include the number of passes through the brushes to remove all arthropods from the leaf, distribution of arthropods on the collection plate, a comparison to direct counts of arthropods on leaves, accuracy and precision of various scanning methods using a dissecting scope, the accuracy and precision of this technique compared to traditional binomial sampling for arthropod pests on grapes, and an analysis of cost-benefits based on the time required to perform the protocol compared to direct counting and binomial sampling.



Species 1: ACARI Tetranychidae Eotetranychus willamettei (Willamette spider mite)
Species 2: ACARI Phytoseiidae
Keywords: sampling, pest management