The recent invasion of Southern California by the glassy-winged sharpshooter (GWSS), Homalodisca coagulata (Say) (Hemiptera: Cicadellidae), has triggered an enormous effort to control the vector of Pierce’s Disease. Long-term, effective control of GWSS will include the exploitation of the pest’s natural enemies. Unfortunately, very little information is available on GWSS’s predaceous natural enemies. Our approach to identify key predators is to use GWSS-specific monoclonal antibodies and DNA probes to detect prey remains in the guts of field-collected predators. We have developed GWSS-specific ELISAs (enzyme linked immunoassay) and PCR (polymerase chain reaction) assays for various lifestages of GWSS. We conducted laboratory tests to determine how long GWSS antigen and DNA can remain detectable in predator guts. Currently, we are assaying field-collected predators from areas infested with GWSS to identify the key enemies. Our ultimate objective is to utilize this information to implement conservation or augmentation biological control programs for GWSS.