Wednesday, 17 November 2004 - 9:36 AM
1002

Chemical identification of host and blood odors that attract mosquitoes

Ulrich R. Bernier, ubernier@gainesville.usda.ufl.edu, Sandra A. Allan, sallan@gainesville.usda.ufl.edu, and Daniel L. Kline, dkline@gainesville.usda.ufl.edu. USDA-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, 1600 SW 23rd Drive, Gainesville, FL

Mosquitoes locate a bloodmeal using host odors as cues for attraction, and possibly these are the primary cues. Vectors of West Nile virus, such as Culex quinquefasciatus and Cx. nigripalpus tend to prefer avian hosts for bloodmeals; therefore, examination of host odors from chickens may provide some of the host-seeking chemical cues used by these mosquitoes. Solvent-based extraction of host emanations using hexane, methanol, and diethyl ether allowed us to concentrate and compare compounds collected from chicken skin, feet, and feathers for subsequent identification by gas chromatography/mass spectrometery (GC/MS). In addition to solvent extraction, collection of volatiles was accomplished using an impinger with diethyl ether, and these results are compared to those observed with solvent extraction methods. In the laboratory,Culex spp. are reared on blood encased in a membrane. It is suspected that volatile cues play a role in the ability to locate the blood for feeding. Therefore, the methodologies described above and used for host emanation analysis were expanded and adapted for collection of volatiles from chicken and bovine blood to identify potential feeding attractants.



Species 1: Diptera Culicidae Culex quinquefasciatus (Southern House Mosquito)
Species 2: Diptera Culicidae Culex nigripalpus
Species 3: Dipter Culicidae Aedes aegypti (Yellow Fever Mosquito)
Keywords: host-seeking, attractants

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