The species Chrysomya chloropyga (=Chrysomya putoria) (Wiedmann) is a synanthropic blowfly native from African regions. The adults feed and breed in carrion, feces, urban garbage and carcasses. Due to these biological features, C. chloropyga have great medical-veterinary importance acting as a vector of pathogens and is considered of potential forensic importance. This fly was probably introduced into Brazil around 1975 with the influx of Angolan refugees. Since then it has spread rapidly in Brazil and other countries of South America affecting the ecological scenario of the native Calliphoridae fauna. Genetic studies based on mitochondrial DNA revealed insufficient polymorphism at a fine scale. Since microsatellites are one of the most powerful markers for studying inter- and intrapopulation relationships, our goal is to develop a set of polymorphic microsatellite markers for C. chloropyga. A genomic library enriched for the motifs poli-AC/TG and poli-AG/TC was constructed. We are currently screening this library for microsatellite-containing clones. Of 54 clones inserts already sequenced, 26 had a microsatellite and 12 are useful sequences for designing primers. After polymorphism evaluation, these loci should contribute to population dynamics studies and for understanding the evolutionary context of the introduction of this species in the New World.