Abstract: A laboratory rearing protocol with artificial diet was developed for the predacious ground beetle, Poecilus chalcites Say (Coleoptera: Carabidae). With the developed protocol, P. chalcites can be reared from eggs to adult for a single generation in the laboratory and used for feeding studies to evaluate the effects of non-contact insecticidal substances (e.g., Bt Cry proteins and potassium arsenate). The usefulness of the diet exposure system for testing P. chalcites was demonstrated with an estimation of the LC50 value of potassium arsenate for both larvae and adults. The methodology for P. chalcites dose response can be adapted to evaluate Bt proteins that are incorporated into transgenic crops.