Geographic distribution, seasonal abundance, and species composition of mosquitoes breeding in discarded tires were determined by larval sampling in sixty-seven counites in Alabama. Two tire sites were sampled in each county twice a month from May to October, 2004. Each larval sample was identified to species. Habitat segregation and species co-existence were determined by larval sampling and adult trapping at two large rural tireyards in Lee and Talladega County.