The house fly, Musca domestica L., is a synanthropic species with worldwide distribution. Due to their unrestricted movement, mode of feeding, developmental habitats, and attraction to places where food is prepared and stored, house flies greatly amplify the risk of human exposure to food-borne and other pathogens. Flies that develop in animal productions where antibiotics are frequently used for prophylaxis and growth promotion carry antibiotic-resistant bacteria that maybe transmitted to food intended for human consumption, leading to illnesses that are difficult to treat. However, no studies have been undertaken to quantify the extent of house fly movement between rural to urban areas. Therefore, our objective is to examine the population genetics of house flies and estimate the extent of dispersal/migration from rural to urban areas and vice versa in Kansas. As a first step, we developed polymorphic microsatellite loci for house flies. Here we report on the isolation, characterization, and optimization of microsatellite loci and preliminary results on population dynamics of house flies.