An easy to use ingestion bioassay-kit to obtain baseline and monitoring susceptibility data for indoxacarb was developed by Dupont in 2000. The kit consists of 16 45-ml wells plastic trays and covers, pre-cut germination paper, forceps, razors, nitrile gloves, a volumetric tube and 200-ml high-density polyestyrene bottles/caps with a hydrateable dose-film of indoxacarb plus FD&C blue #1dye and 500 ppm of the adjuvant X-77. Slight variations of the kit are also discussed. A customized Microsoft Excel-based log dose response add-in for data collection and analysis and an electronic training module were also developed. Insects and plant parts collected from the field are used for the assay. A predetermined amount of water is added to the treated bottles and shaken until the dye is completely dissolved indicating that the indoxacarb film is in complete suspension at the pre-set concentration. Plant parts are immersed in the bottles, allowed to dry and a piece is placed in each tray-cell along with a moistened piece of germination paper. One test insect is placed per cell, the tray is covered and kept at room temperature. Evaluation of insects that are dead, moribund and alive, and percent feeding damage (0-100 scale) is made 72 hours after setup. The kits have been used to develop baseline data and monitor susceptibility to indoxacarb on Helicoverpa armigera, Heliothis virescens, Spodoptera exigua, S. eridania, Tuta absoluta, Plutella xylostella, Lobesia botrana, Cydia pomonella, and others. Diagnostic doses have been determined on selected pest species and are being implemented.