While collecting insects in various habitats in Florida, we found mites associated with four insect orders, and in some cases they were quite prevalent. In Orthoptera, a mite belonging to the genus Leptus was found parasitizing the grasshoppers Eritettix obscurus (Scudder), Melanoplus keeleri (Thomas), Melanoplus ordwayae Deyrup, and Melanoplus rotundipennis Scudder. From the order Coleoptera, a parasitic mite was found on the dung beetle Phanaeus vindex MacLeay. Mites collected on the carrion dwelling beetles, Dermestes caninus Germar and Oiceoptoma inaequale (Fabricius), as well as the Mexican bromeliad weevil (Metamasius callizona (Chevrolat)) seemed to have a phoretic relationship. Two mosquitoes, Anopheles crucians (Wiedemann) and Uranotaenia sapphirina (Osten Sacken) (Diptera), were parasitized by two separate species of mites. Also, the giant water bug Lethocerus sp. (Hemiptera: Belostomatidae) was carrying parasitic mites. Mite specimens were slide-mounted to be identified, and were sampled for molecular analysis.