The invasive Soybean aphid, Aphis glycines, (Hemiptera: Aphididae) was first sighted in the United States in July of 2000, and threatens soybean production. Previous to this invasion there were few aphids feeding on soybean. Our objective was to detect the presence of resident parasitoids and predatory flies that have adapted to utilize soybean aphid. Soybean aphid natural enemies were sampled three times, once in June, once in August and once in September. Pots of soybean infested with soybean aphid were exposed to parasitoids and predators for a three day period in different cropping system treatments. Parasitoids oviposited in aphids and predatory flies oviposited on aphid-infested soybean foliage of the pots. Parasitoid and predatory fly progeny were then reared and identified. Ten species of native or previously introduced aphid natural enemy attacking soybean aphid, including three species of braconid, two aphelinids, three species of syrphid, a cecidomyiid and a chamaemyiid were detected. Their seasonal and cropping system occurrence is presented.