Colonies of the red imported fire ant, Solenopsis Invicta Buren, were collected from New Orleans, Louisiana, city park and placed in plastic boxes coated with Fluon 3.8 cm from the top of the box to contain the ants. Half of the colonies were provided with a nutritionally balanced bait formulation and 5% boric acid (Drax NutraBat). The rest were provided with the bait formulation deprived of boric acid. Ant colonies were kept at room temperature (25 ± 5°C) for 5 days. Venom was extracted from randomly selected ant workers using two methods. The first method consisted collecting venom droplets using 1-ìl capillary tubes with the aid of a stereo microscope. Venom of 15 ants from each treatment was dissolved in 30 ìl of hexane. In the second method venom was extracted by dissecting the ovipositor containing venom gland. Venom glands of 5 ants from each treatment were placed in 30 ìl of hexane and crush while stirring using a 1- ìl syringe piston. This mix was filtered to eliminate solid residuals. Both methods of extraction were repeated 3 times. The extracted solutions were analyzed using a GC/MS system. Five peaks were present in the chemical analysis, two of which were identified as solenopsin A and solenopsin B. Concentrations of these 2 compounds were more than 3 times higher in the control treatment than in the boric acid treatment. The effect of boric acid on the venom composition of S. invicta and the mechanisms of interference of venom synthesis are discussed.