The Indian meal moth, Plodia interpunctella Hübner, is a serious and widespread pest of stored food commodities throughout the world. The moth is resistant to most of the insecticides used to control it. Proper understanding of the breeding structure, dispersal, migration, gene flow, and movement of resistant alleles has great implications for management of this pest. Therefore, we have initiated an investigation of the population genetics of Indianmeal moth worldwide. As a first step, we developed polymorphic microsatellite markers from genomic DNA enriched for microsatellite loci. These loci were amplified across populations from different parts of U.S. and around the world. Preliminary results give an insight into the population structure, dispersal, genetic relationship and extent of gene flow among the sub populations studied. These markers are an invaluable tool for population genetic studies of Indianmeal moth and can be used in DNA fingerprinting for population level identification of this pest.