The effects of the microsporidium Thelohania solenopsae on host fat body tissues and cells of alates and dealates of the red imported fire ants were investigated with regard to apoptotic activities using annexin V stain. In the first experiments none of the cells was found to undergo necrosis as all of the infected cells were either apoptotic or viable. In the next experiment, to investigate whether or not infected tissues and cells were resistant to apoptotic inducers, staurosporine was used to induce apoptosis. The results indicated that, at the tissue level, interaction between time and the inducer was the only significant predictor of differences in percentage of apoptosis. The percentage of infected cells could not explain the differences in the percentage of apoptosis between infected and uninfected tissues. When comparing between infected and uninfected cells, the results suggested that staurosporine was effective in inducing apoptosis in both infected in uninfected cells. Also, there were a significantly higher percentage of infected cells undergoing apoptosis than uninfected cells. These results did not support the idea that microsporidia infection may inhibit apoptosis. Instead, these results supported the idea that infection with microsporidia may induce apoptosis in the case of T. solenopsae infection with the red imported fire ants.