The diversity of bark beetles (Coleoptera: Curculionidae: Scolytinae) from multiple sites in the Ecuadorian rain forest canopy is presented. Beetles collected by insecticide fogging were sorted to morpho-species and identified to the lowest possible taxonomic level. Bark beetle diversity was compared among collecting sites. Biodiversity at each site was also associated with vegetation type. Species accumulation curves were calculated for each site to estimate the amount of collecting effort needed to arrive at an accurate representation of the bark beetle fauna.