Method for the extraction and analysis of imidacloprid residues in plant material by Enzyme-Linked Immunosorbent Assay (ELISA)
John Molongoski, email@example.com, Jessica Hagan, firstname.lastname@example.org, and Phillip A. Lewis, email@example.com. USDA APHIS-PPQ, Bldg 1398 W. Truck Rd, Otis ANGB, MA
Imidacloprid residues in plant material are typically determined by extraction of the sample in an organic solvent followed by analysis of the pesticide residue by either High Pressure Liquid Chromatography (HPLC) or by Gas Chromatography-Mass Spectrometry (GC-MS). These methods, while accurate, are expensive and time consuming to perform on large numbers of samples. An Enzyme Linked Immunosorbent Assay (ELISA) is commercially available which can analyze large numbers of samples with great sensitivity and at reduced cost. However, this assay is currently restricted to the determination of imidacloprid residues in ground and surface water samples. We attempted to develop a technique whereby the ELISA assay could be utilized to determine imidacloprid residues extracted from the leaves, wood, and bark of imidacloprid-treated maple and ash trees. Numerous solvent extraction schemes to remove the imidacloprid residues from the plant tissue were tested and the tolerance of the ELISA assay to these various extraction techniques is described. Imidacloprid was extracted both from laboratory spiked and field injected plant tissue. Residue levels obtained by tissue extractions were compared to imidacloprid levels in xylem sap squeezed from leaf bracts of the same trees.
From i.matusik, January 10, 2006 Dear Sir or Madame,
I'm Polish chemistry student fom Silesian University and I'm writing toYou in special case.We analyze together with biological faculty vaccinefor Cameraria ohridella the active component of which is imidacloprid. Iknow, that You have great experience in studies like this ( I mean extraction from plant material). From prepared to analysis leaves, wewant to isolate imidacloprid and make marking using HPLC method.Our mainproblem is to remove chlorophyll without losing imidacloprid from oursample in the same time. I would be very greatfull if you send me somematerials,links or articles (about extraction from green plant, andchlorophyll isolation). Maybe You know someone who is trying to solvethe same problem.Yours faithfully,
From pushpinder kaur, Good day!, CEU, September 10, 2006 Dear Sir,
I have the same problem as Izabela of Selisian University has...please send me also some link or article or procedure of isolating imidacloprid. thank you!
From francis oppong, Mr, University of Ghana, October 10, 2006 I currently carring out a project on imidacloprid residues that may present in cocoa pod. i will like to know if the imidacloprid residues can be isolated by paper or column chromatography
From harish kumar , December 8, 2008 sir,
please tell me how do the marking in hplc. how find the result in sftware of lc solution.