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Tuesday, 16 November 2004
D0347
Inheritance of resistance to Bacillus thuringiensis in cabbage looper, Trichoplusia ni
Jian-Zhou Zhao, jz49@cornell.edu1, Ping Wang, pw15@cornell.edu1, Wendy C. Kain, wck2@nysaes.cornell.edu1, Anthony M. Shelton, ams5@cornell.edu1, Alida F. Janmaat, janmaat@zoology.ubc.ca2, and Judith Myers, myers@zoology.ubc.ca2. (1) Cornell University / NYSAES, Dept. of Entomology, 630 W. North Street, Geneva, NY, (2) University of British Columbia, Department of Zoology, 6270 University Boulevard, Vancouver, British Columbia, Canada
A population of cabbage looper, Tricoplusia ni, collected from commercial greenhouses in the lower mainland of British Columbia, Canada in 2001 showed a resistance level of 7.8 fold to Dipel, a product of Bacillus thuringiensis (Bt) subsp. kurstaki. After seven generations of selection of this population with a Bt protoxin (Cry1Ac), we obtained a T. ni strain, named GLEN-Cry1Ac, highly resistant to Cry1Ac with a resistance ratio (RR) of 981-fold. The larvae from the GLEN-Cry1Ac strain could survive on Cry1Ac-expressing transgenic broccoli plants. The inheritance of Cry1Ac resistance in this T. ni strain was autosomal, incompletely recessive and monogenic. Evident cross-resistance to Cry1Ab and Cry1F was observed by using two near-isogenic strains of T. ni (RR=870-fold to Cry1Ac).
Species 1: Lepidoptera Noctuidae Trichoplusia ni (cabbage looper)
Keywords: Bacillus thuringiensis, resistance
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