The Colorado potato beetle, Leptinotarsa decemlineata (Say), is the most destructive insect pest of potato, Solanum tuberosum (L.) in eastern North America. The insect has adapted to every insecticide used to manage it. Avidin is a protein found in chicken egg whites that has demonstrated insecticidal properties against a number of Lepidopteran and Coleopteran pests. This protein protects the chicken embryo by sequestering biotin from disease causing organisms. Biotin is an essential co-enzyme required for all organisms, including insects. Biotin is a cofactor of a carboxylase which is required for many important processes like lipogenesis, gluconeogensis, fatty acid and amino acid catabolism. Without this co-enzyme, an insect’s growth is severely stunted, eventually leading to death. The gene for avidin production has been cloned and inserted into a few crops, including maize, tobacco and potato and has demonstrated resistance to a wide spectrum of insect pests. We have expressed avidin in two potato lines: MSE149-5Y, a susceptible potato line, and ND5873-15, a high glycolakaloid line. Detached leaf bioassays were performed on transgenic and non-transgenic clones of MSE149-5Y and ND5873-15 using Colorado potato beetle neonates and third instars. Survivorship and consumption were measured every 2d over a 12d period for neonates and every 2d over a 6d period for third instars.