Methods for evaluating ovarian development and mating status in the olive fruit fly (Bactrocera oleae (Gmelin)) for use in tracking reproductive phenology
Hannah Joy Burrack, email@example.com, Angela M. Fornell, and Frank G. Zalom, firstname.lastname@example.org. University of California, Davis, Department of Entomology, One Shields Avenue, Davis, CA
Five basic stages of ovarian development for the olive fruit fly (Bactrocera oleae (Gmelin)) were determined as follows:
1. No distinct ovarioles visible. Ovaries appear frothy. Pre-oviposition period.
2. Only immature ovarioles visible. Pre-oviposition period.
3. Ranging from immature ovarioles to mature eggs. Oviposition period.
4. Only mature eggs visible. Oviposition period.
5. No ovarioles visible. Ovaries do not appear frothy. Post-oviposition period.
These stages were then used to rate female olive fruit flies which were collected throughout California during the entire year. Mating status was determined by removing both spermatheca and crushing them in 1 drop of 1x DAPI stain in phosphate buffer solution (PBS) on a microscope slide. This general DNA stain stains sperm more intensely than surrounding tissue. Sperm presence or absence was determined through observation using florescence microscopy. Data collected using these methods are being used to track reproductive phenology of the olive fruit fly throughout the state of California and to confirm generation timings observed through monitoring trap captures.
Species 1: Diptera Tephritidae Bactroceraoleae (olive fruit fly) Keywords: fluorescence microscopy