Monday, 15 November 2004
D0076

Spatial distribution of volatiles produced by wheat plants infested with wheat stem sawfly Cephus cinctus (Hymenoptera: Cephidae), and of topically applied chemicals to healthy wheat plants

Oscar G. Perez, operez@montana.edu, David K. Weaver, weaver@montana.edu, Perry R. Miller, pmiller@montana.edu, and Wendell L. Morrill, wmorrill@montana.edu. Montana State University, Department of Entomology, Bozeman, MT

The present work is part of a research program intended to find a method to enhance the attraction and detection of parasitoids (Bracon cephi and Bracon lissogaster) to wheat stem sawfly larvae feeding inside wheat stems. The wheat steam sawfly is a very difficult pest to control and the use of parasitoids is a promising control method. The distribution of the chemical volatiles produced by infested wheat plants was studied. The experiments were conducted in the field and under controlled greenhouse conditions. Wheat plants at different densities were planted every two weeks during a seven month period. The plants were infested with wheat stem sawflies after reaching the development stage of Zadoks 35. Volatiles were collected from the infested plants with a portable volatile collecting system (VCS) at four different plant heights and three different distances. The collected volatiles were analyzed using a gas chromatography-mass spectrometry system (GC-MS) and analyzed to determine the spatial distribution of the volatiles produced by the infested plants. A second study under the same conditions as the first one was conducted to determine the spatial distribution of the volatiles produced by topically applying vanillin and camphor to healthy wheat plants. Based on the release rates obtained with these chemicals using a VCS system, low concentration solutions of vanillin and camphor were prepared and topically applied to healthy plants. Volatiles were collected from these plants and analyzed using the procedure described above.


Species 1: Hymenoptera Cephidae Cephus cinctus (Wheat stem sawfly)
Species 2: Hymenoptera Braconidae Bracon cephi
Species 3: Hymenoptera Braconidae Bracon lissogaster
Keywords: gas-chromatography, Zadocks

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