Monday, 15 November 2004 - 8:42 AM
0117

Green peach aphid responses to volatiles produced by potato leafroll virus-infected potatoes at various times following inoculation

Brent J. Werner, wern7078@uidaho.edu1, Thomas M. Mowry, tmowry@uidaho.edu2, Sanford D. Eigenbrode, sanforde@uidaho.edu1, Nilsa A. Bosque-Perez, nbosque@uidaho.edu1, and Hongjian Ding, hding@uidaho.edu1. (1) University of Idaho, Department of Plant, Soil, and Entomological Sciences, Moscow, ID, (2) University of Idaho, Department of Plant, Soil, and Entomological Sciences, Parma Research and Extension Center, Parma, ID

Green peach aphid emigration rates were measured by observing apterous aphids presented with headspace volatiles from intact potato plants over the course of PLRV disease progression. A repeated measures design was implemented to observe this response to volatiles from potato plants 2, 4, 6, 8, and 10 weeks following inoculation with PLRV. In addition to the PLRV-infected potatoes, other treatments included noninfected plants sham-inoculated with virus-free aphids, uninoculated plants, and a soil-filled pot. Tests were conducted with thirty aphids being placed over a 4 cm diameter hole on a platform with a screen floor which was suspended over an entire potato plant. Observations were recorded continuously for 60 minutes by marking the time that each aphid left the area prescribed by the hole in the platform. Aphids were considered to have emigrated as soon as they left his area and were not allowed to return. Following the bioassay, volatiles were collected from all treatment plants and leaf samples collected for measuring PLRV titer using quantitative DAS-ELISA. Emigration rates were compared among treatments and related to their respective volatile profiles and virus titers.


Species 1: Homoptera Aphididae Myzus persicae (green peach aphid)
Keywords: virus-induced volatiles

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