Tuesday, 16 November 2004

Nutrigenomics of the predatory pentatomid Perillus bioculatus

George D. Yocum, yocumg@fargo.ars.usda.gov, ARS-USDA, Insect Genetics and Biochemistry Research Unit, Biosciences Research Laboratory, 1605 Albrecht Blvd, Fargo, ND and Thomas A. Coudron, coudront@missouri.edu, USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Rd, Research Park, Columbia, MO.

Currently, artificial diet development is a hit or miss proposition due to having to rely on life history parameters to direct diet formulation efforts. This black box approach does not provide insight as to exactly how to resolve the problem(s) with the diet. The new field of nutrigenomics holds the promise of being able to identify specific dietary deficiencies enabling rapid development of an optimized diet. We have screened 1300 clones from Perillus bioculatus fed either host or an artificial diet and isolated 92 independent dietary regulated clones. Transcripts for enzymes involved in tyrosine and glucose metabolism were upregulated in nymphs fed on the suboptimal artificial diet. Nymphs fed on different formulations will be screened with these clones to direct diet modifications in an effort to optimize the artificial diet.

Species 1: Hemiptera Pentatomidae Perillus bioculatus (Two-spotted stink bug)
Keywords: artificial diet

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