We postulated that An. gambiae females would not oviposit onto moving water, and that larvae would not be able to feed nor grow in a current. In a three choice experiment (moving water and two still water controls), An. gambiae females laid more eggs on moving water compared to either still water control, by a factor of three. Although larval survival over a one-week period was lower in moving water than in still water or in still water where larvae were starved, larvae grew to pupation in moving water when provided food. Larvae that were starved did not pass the second instar. The number of pupae eclosing from moving water was lower than that eclosing from still water, but the body size was the same between adults eclosing from these treatments. There was no difference in the female to male ratio in moving versus still water. When pupae were introduced into a rapidly spinning vortex, 30 of 100 eclosed successfully as adults. This laboratory study indicates that, for An. gambiae, moving water is not inhibitory to oviposition, permits larval growth, and after accounting for survival, does not impair fitness of emerged adults.