Field and laboratory experiments were conducted during 2003 and 2004 to determine the effectiveness of 2 predatory mite species, Phytoseiulus persimilis Athias-Henriot and Neoseiulus californicus McGregor, and a reduced-risk miticide, Acramite 50WP®, for control of twospotted spider mite (TSSM) (Tetranychus urticae Koch) in North Florida strawberry fields. In field studies N. californicus significantly reduced populations of TSSM below numbers recorded in untreated (control) and P. persimilis plots. Acramite was also effective, significantly reducing TSSM populations. Similar trends were seen in laboratory experiments. The results advocate the use of N. californicus and Acramite 50WP® in strawberry fields in north-central Florida.