Wednesday, 17 November 2004
D0465

Random amplified polymorphic DNA (RAPD) based molecular characterization of scarab beetles (Scarabaeidae: Coleoptera) from Pakistan

Muhammad Kashif Zahoor, kashif_uaf@hotmail.com and Anjum Suhail, dranjumsuhail@hotmail.com. University of Agriculture, Entomology, Insect Biodiversity & Biosystematic Lab, Faisalabad, Punjab, Pakistan

The family Scarabaeidae presents remarkable species diversity and striking morphological and biological variation. Some representatives of this family were selected for the assessment of genetic polymorphism by using random amplified polymorphic DNA (RAPD) technique. A total of 15 short oligonucleotide primers of arbitrary sequence were used in the PCR reaction. The amplified products were resolved on agarose gel electrophoresis. One hundred and thirty five bands were observed with an average of 9.0 bands per primer. Of these amplified bands almost all the fragments showed polymorphism. Adoretus bimarginatus produced the maximum number of DNA amplified fragments while minimum number was produced by Catharsius pithecius. The genetic polymorphism was high, ranging from 44 % to 71 %. The data generated through these amplified bands were analyzed to construct the dendrogram using unweighted paired group method of arithmetic means (UPGMA).


Keywords: RAPD

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