The potential of commercially available air curtains to prevent mosquitoes and flies from entering aircraft was studied in two passenger boarding bridge (jetway)/aircraft mock-up structures. Air curtains were mounted in the jetway door contiguous to the aircraft door in selected configurations. Three species of mosquitoes, Aedes aegypti (L.), Anopheles quadrimaculatus Say, and Ochlerotatus taeniorhynchus (Wiedemann) and house flies, Musca domestica L., were released in the opposite end of the jetway after air curtains were activated. Personnel then made 25 passes through the jetway past the air curtains and into the aircraft to simulate passenger boarding. After the last pass, a door was closed to separate the jetway from the aircraft, and mosquitoes and flies in the aircraft were counted and identified to species. The air curtains effectively excluded mosquitoes and flies, 99 and 100% respectively, from passing through the doorways that had human traffic similar to passengers boarding an aircraft.