Daniel Kessete Ghebreab, 200148303@ctech.ac.za1, Michael Tobin, Mtobin@uwc.ac.za2, and Sehaam Khan, khans@ctech.ac.za1. (1) Cape Technikon, Health Sciences, Cape Town 8000, Cape Town, South Africa, (2) University of Western Cape, Biotechnology, UWC, Bellville 7535, Cape Town, South Africa
Baculoviruses were of the first viruses shown to
modify the host apoptotic response. Apoptosis is a
strictly regulated and evolutionary conserved
mechanism by which a cell self-destructs. This
condition is characterised by chromatin condensation,
blebbing and DNA fragmentation. The process of
apoptosis has an important impact on the evolution of
the viruses and their strategies of replication.
Viruses stimulate apoptosis very early in their
replication process, however, baculoviruses have
mechanisms that are able to function in the apoptotic
pathway and prolong the life of the infected cells
until they have completed replication. These viruses
have anti-apoptotic genes (iap) and p35 that suppress
the activity of the host apoptotic response. In this
study one set of iap specific primers was designed to
amplify a 450bp internal region of the iap gene. This
amplicon was used as a probe to hybridise to the SacI
genomic library of
Trichoplusia ni single-enveloped
nucleopolyhedrovirus (TnSNPV). The initial
phylogenetic analysis of this 450bp region of the iap
gene suggests that TnSNPV belong to group II
baculoviruses.
Species 1: Lepidoptera Noctuidae
Trichoplusia ni (cabbage looper)
Species 2: Baculoviridae Eubaculoviridae (
Trichoplusia ni single enveloped nucleocapside virus(TnSNPV), Nucleopolyhedroviruses)
Species 3: Lepidoptera Noctuidae
Autographa californica (alfalfa looper)
Keywords: Apoptosis
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