Ecdysone receptor (EcR) and its heterodimer, ultraspiracle protein (USP), are ligand dependent transcriptional factors. Transcriptional factors are often regulated by phosphorylation. In this study, the role of USP phosphorylation in regulating the function of EcR/USP complex was investigated. Phosphorylation site-specific usp mutant clones were generated using site-directed mutagenesis, cloned into pBlueBac4.5 transfer vector, and co-expressed with EcR clone in Sf9 cells using baculovirus expression system. The effects of site-specific USP phosphorylation on protein expression mediated via the EcR/USP complex were examined using 2D-gel differential display technique.
Species 1: Diptera Drosophilidae Drosophila melanogaster
Keywords: Ecdysone receptor, ultraspiracle protein
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