Sunday, 26 October 2003 - 1:36 PM
0164

This presentation is part of : Ten-Minute Papers, Section D. Medical and Veterinary Entomology

Examination of mitochondrial haplotypes of pesticide resistant strains of the cattle fever tick, Boophilus microplus

H. Joel Hutcheson1, John E. George2, Ronald B. Davey3, Felix D. Guerrero2, G. Bradlee Mitchell1, Susan James1, Chris Gorham1, and Brian Kleker1. (1) Colorado State University, Arthropod-borne and Infectious Diseases Laboratory, Foothills Campus, Fort Collins, CO, (2) USDA/ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Road, Kerrville, TX, (3) USDA, ARS, Cattle Fever Tick Research Laboratory, Rt. 3, Box 1010, Edinburg, TX

Pesticide resistant genetic strains of Boophilus microplus and B. annulatus were examined to reveal possible genetic variation in the 16S and 12S regions of the mitochondrial genome. Comparisons using single stranded conformation polymorphism (SSCP) of the approximately 460 bp of the 16S and approximately 400 bp of the 12S regions revealed no variation, i.e., a single mitochondrial haplotype for seven genetic strains of B. microplus, which differ in their resistance to acaricides, in contrast to a single strain of B. annulatus, which revealed two haplotypes in the 3' end of the 16S. These findings suggest that the 16S may not vary in one-host ticks to the same degree as in three-host metastriates or in prostriates.

Species 1: Acari Ixodidae Boophilus microplus (southern cattle tick)
Species 2: Acari Ixodidae Boophilus annulatus (cattle tick)
Keywords: SSCP, PCR

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