We identified a 30 bp juvenile hormone (JH)-response region containing direct repeat elements (JHRE) in the promoter region of JH esterase gene. In Drosophila melanogaster L57 cells, a reporter gene placed under the control of this JHRE was induced by JH I in a dose- and time-dependent manner. The nuclear proteins isolated from L57 cells specifically bound to JHRE. To identify genes involved in JH-response, we performed microarray analysis. The GeneChip® Drosophila Genome Array containing probe sets for 13,500 genes and RNA isolated from L57 cells grown in the presence of 1 µM JH I for 0, 3, 6 and 12 hr were used to analyze gene expression. Out of 13,500 genes tested, the expression of only 8,000 genes was detected in at least one of the samples. Statistical analyses were performed on four independent data sets at each time intervel, only genes that showed the p value of <0.05 were included in the analysis. The cells that were exposed to JH I for 3 hr showed the maximum changes in gene expression, in these cells, 337 genes showed an increase (1.5 to 6.93-fold) and 98 genes showed a decrease (1.5 to 4.4-fold) in expression when compared to the cells grown in the medium containing DMSO. Northern blot hybridization and quantitative reverse transcriptase polymerase chain reaction are being used to confirm the expression profiles of some of the induced and suppressed genes.
Species 1: Diptera Drosophilidae Drosophila melanogaster (Fruit fly)
Keywords: Juvenile hormone, Microarray
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