Tuesday, 28 October 2003
D0261

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Molecular characterization of Hessian fly, (Mayetiola destructor) salivary secretary cDNAs with structural similarity to protease inhibitors

Ashoka A Maddur1, Xuming Liu1, John P. Fellers2, Gerald E. Wilde1, and Ming-Shun Chen1. (1) Kansas State University, Entomology, 123 Waters Hall, Manhattan, KS, (2) Kansas State University, Plant Pathology, Throckmorton Hall, Manhattan, KS

Hessian fly, Mayetiola destructor (Say), is one of the major pests of wheat, which cause enormous economic loss. Several lines of evidence suggest that Hessian fly larvae inject saliva into host plants during feeding and cause severe stunting and plant death at seedling stage. To identify components injected into host plants by the Hessian fly, we adopted an EST approach to identify and characterize cDNAs and genes that encode proteins with a secretion signal peptide. BLAST nucleotide similarity searches indicated that two of the identified clones code for protease inhibitors. Northern blot data shows that the identified clones were found to be expressed only at first instar larvae which is critical for the larvae to establish infestation.

Species 1: Diptera Cecidomyiidae Mayetiola destructor (Hessian fly)
Keywords: Expressed Sequence Tag

Back to Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology
Back to Posters

Back to The 2003 ESA Annual Meeting and Exhibition