Abstract: A major problem encountered with development of biopesticide formulations using entomopathogenic organisms is exposure to UV-light. We have been developing a fluidized bed process to entrap Metarhizium anisopliae var acridum spores in a lignin-based matrix that will provide protection from the damaging effects of UV-light. The entrapment parameters such as pH and temperature influenced the particles size distribution of entrapped spores. The granule size and ratio of the fluidized bed drying matrix components influenced the product moisture content and particle size distribution. The entrapped and dried spores had a moisture content of 5%, particle size less than 35 µm with an average of one spore per particle. Spore germination at 24 h was 88%, compared to 96% for uncoated spores. After 48h, the germination of coated spores was comparable to uncoated spores. Bioassay results showed that the entrapped spores were capable of germinating and infecting hosts.
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